Mohamed, et al (2024) Synergistic effects (adsorption and biodegradation) of Streptomyces hydrogenansimmobilization on nano-reed biochar for further application in upflow anaerobic sludge blanket. RSC Advances. https://doi.org/10.1039/D4RA02864C


Water pollution from wastewater, particularly from organic compounds, is a critical environmental issue. This study explores a sustainable method using Phragmites australis biochar combined with the bacteria Streptomyces hydrogenans to treat wastewater from a buffalo farm. The biochar serves as a substrate for microbial colonization and supports microbial growth, while the immobilized bacteria enhance the degradation of organic matter.

Advanced techniques such as infrared spectroscopy, scanning electron microscopy, X-ray diffraction, and BET analysis were used to characterize the biochar and evaluate its performance. The study used a factorial design to optimize the conditions for maximum efficiency, focusing on biochar dose, buffalo sludge dose, pH, and bacteria type.

Results demonstrated a significant improvement in Chemical Oxygen Demand (COD) removal efficiency and biogas production. The optimal conditions—20 g/L biochar, no buffalo sludge, pH 7.5, and Streptomyces hydrogenans—achieved a COD removal efficiency of 92.7% and biogas production of 5.0 mL. The study aligns with the United Nations Sustainable Development Goals 6 (clean water and sanitation) and 12 (responsible consumption and production), indicating its potential for large-scale application in wastewater treatment.

By utilizing locally available materials and environmentally friendly processes, this approach presents a promising solution for improving wastewater treatment and supporting sustainable development.


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