The use of soil amendments is a promising approach to remediate radioactive nuclide contamination. A recent study tested different levels of biochar to clean uranium-contaminated soil in potted plants. Adding 1% biochar yielded the best results, deactivating uranium, increasing soil enzyme activity, and promoting ryegrass growth. Microbiological and metabolomic analysis revealed that 1% biochar significantly boosted the abundance of microorganisms like Actinobacteriota and Myxococcota, and accelerated the production of metabolites such as lipids, organic acids, and organic oxygen compounds. Analysis showed that the interaction between bacteria, enzymes, and metabolites improves the expression of the ABC transporter’s metabolic pathway, enhancing cell resistance to uranium and maintaining homeostasis under uranium stress. This study exemplifies biochar-assisted phytoremediationThis is a technique that uses plants to clean up contaminated soil or water. Biochar can enhance phytoremediation by improving soil conditions and promoting plant growth, allowing plants to absorb and break down pollutants more effectively.   More and provides theoretical guidance for remediating soil contaminated with radioactive nuclides.