Maintaining soil quality is paramount for sustainable agriculture, especially in valuable mollisol regions that are critical for global food production but face degradation due to intensive farming practices. A recent study published in Agronomy by Likun Hou et al., investigates the impact of corn straw biochar on mollisol soil quality and maize yield within a maize-soybean rotation system. The research provides valuable insights into how biochar application methods affect soil properties and crop productivity, emphasizing the critical role of physical and biological indicators.

Mollisols, known for their high fertility, have experienced significant degradation, with topsoil thinning by 30-40 cm since the 1950s in Northeast China, a region vital for the country’s grain reserves. This degradation is characterized by increased soil bulk density, decreased total porosity, and a rapid decline in soil organic matter (SOM). Crop rotation and organic amendments like biochar are recognized as effective strategies to mitigate these issues. Biochar, with its high surface area and porosity, improves water and nutrient retention, reduces soil bulk density, and enhances soil structure. This study aimed to quantify these benefits in a maize-soybean rotation.

The researchers established two biochar application methods: biochar mixed in 0-20 cm soil depth (B1) and biochar mixed in 0-40 cm soil depth (B2), comparing them to a control (CK) without biochar. After three years of maize-soybean rotation, soil samples were collected to assess physical, chemical, and biological properties, as well as crop yields.

The results demonstrated significant improvements in soil quality with biochar application. Compared to the control, biochar reduced soil bulk density by 3.1% and electrical conductivity by 19.5-28.25%. Soil organic matter content increased by 7.2%, ammonium nitrogen by 6.7-12.0%, available nitrogen by 6.7-18.5%, available phosphorus by 15.6-23.8%, and available potassium by 11.6-17.3%. Biochar also boosted soil enzyme activities: urease activity by 12.25-21.6%, sucrase activity by 6.8-30.8%, neutral phosphatase activity by 5.6-9.7%, and catalase activity by 13.6%.

A minimum data set (MDS) for soil quality assessment was established using principal component analysis, selecting four key indicators: bulk density, water content, pH, and nitrate nitrogen. The soil quality index (SQI) was calculated based on these indicators, showing a significant increase of 14.6-63.3% with biochar application compared to the control. Specifically, in 2021, B1 and B2 increased SQI values by 25.0% and 14.6%, respectively. In 2022, these effects were even more pronounced, with B1 and B2 increasing SQI values by 50.0% and 63.3%, respectively. This suggests that while B1 (0-20 cm depth) had a quicker short-term impact, B2 (0-40 cm depth) showed a more profound and sustained influence on soil quality over time, likely due to slower decomposition in deeper soil and continuous nutrient release.

Crop yields also saw notable benefits. Biochar application significantly increased maize yields by 5.6-7.2%. In 2021, B1 and B2 increased yield by 7.2% and 6.7%, respectively. In 2022, B1 and B2 led to yield increases of 5.6% and 5.7%. The study found a strong positive correlation between SQI and crop yield, indicating that improved soil quality directly contributed to higher productivity.

Further analysis using random forest and partial least squares structural equation modeling revealed that biological indicators, particularly soil catalase activity, were significantly correlated with crop yields across all treatments. This suggests that biochar primarily enhances soil quality and maize yield by reducing soil bulk density—which promotes root and microbial respiration and nutrient supply—and by increasing the activity of soil catalase, an enzyme crucial for nutrient release and absorption.

The study concludes that the interaction between rotation systems and biochar application effectively improves mollisol soil quality. While immediate benefits were seen with shallower biochar incorporation, the long-term advantages of mixing biochar in the 0-40 cm soil layer appear more promising for sustainable farmland utilization. Future research could further explore the impact of biochar on soil microbial indices for a more comprehensive understanding.


Source: Hou, L., Wang, Y., Wang, Z., Gao, R., Zhou, X., Yang, S., Luo, X., Jiang, Z., & Liu, Z. (2025). Effects of Biochar on Soil Quality in a Maize Soybean Rotation on Mollisols. Agronomy, 15(5), 1226.


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