Hamada, K., Nakamura, S. & Kuniyoshi, D. Pipe experiment elucidates biochar application depth affects nitrogen leaching under crop present condition. Sci Rep14, 22823 (2024). https://doi.org/10.1038/s41598-024-73621-3


Biochar is increasingly recognized as an eco-friendly soil amendment with the potential to reduce nitrogen leaching and enhance crop growth. Nitrogen leaching, the loss of nitrogen from fertilizers into groundwater, poses a significant environmental threat, particularly in agricultural regions. However, the effectiveness of biochar in mitigating this issue depends heavily on how deep it is applied in the soil. Recent research has sought to evaluate the relationship between biochar application depth, crop growth, and nitrogen leaching, offering insights into how best to use biochar for sustainable agriculture.

A controlled experiment using pipe-based systems was conducted to examine how different biochar application depths affect nitrogen absorption and water dynamics under crop-growing conditions. Four treatment scenarios were tested: no biochar (control), surface application (0-5 cm depth), plow layer application (0-30 cm), and subsurface application (25-30 cm). Upland rice was used as the test crop in this study, which spanned 91 days.

Key Findings

Surface Application Yields Best Results. Surface-applied biochar significantly improved nitrogen absorption and reduced nitrogen leaching. Nitrate nitrogen (NO₃⁻-N) leaching was reduced by 87.7% compared to the control, while soil-water stress was minimized, leading to better crop growth. The surface application of biochar also enhanced root development, which contributed to more efficient water and nitrogen uptake. These positive effects were attributed to the biochar’s ability to retain moisture in the upper soil layers, promoting better root health and reducing the downward movement of nutrients.

Plow Layer Application Increases Leaching. In contrast, biochar applied in the plow layer (0-30 cm) had an adverse effect. Nitrogen absorption worsened, and both nitrate (NO₃⁻-N) and ammonium (NH₄⁺-N) leaching increased significantly. The plow layer application resulted in higher dry stress in the shallow soil layers, stunting root development and preventing effective nitrogen uptake. This led to increased nitrogen loss through leaching, exacerbating environmental concerns.

Subsurface Application Provides Little Benefit. Applying biochar in the subsurface (25-30 cm) yielded results similar to the control treatment, offering no significant improvement in nitrogen retention or crop growth. This suggests that the subsurface placement of biochar may not be an effective strategy for mitigating nitrogen leaching or promoting crop health.

Water and Nitrogen Dynamics. The experiment also highlighted the role of biochar in altering soil water conditions. Surface-applied biochar reduced soil-water evaporation, helping maintain moisture levels in the deeper soil layers, which is critical for crop growth. Plow layer application, however, led to increased evaporation and a drier upper soil profile, exacerbating stress conditions for the plants.

Root Growth and Nitrogen Uptake. The presence of crops played a crucial role in nitrogen dynamics. Biochar, when applied at the surface, enhanced root growth and nitrogen uptake, leading to a better nitrogen-use efficiency. Conversely, in the plow layer application, poor root growth meant less nitrogen absorption and higher nitrogen leaching.

The study underscores the importance of applying biochar at the correct depth to optimize its benefits. Surface applications of biochar offer the most significant advantages in reducing nitrogen leaching and promoting healthy crop growth. In contrast, deeper applications in the plow layer can increase nitrogen loss, negating the environmental benefits of using biochar. These findings suggest that farmers and land managers should consider biochar application depth carefully to improve nitrogen use efficiency and reduce the environmental impact of agriculture.


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